How it works
band
ladder
selected
- Analyze the membrane.
- Review bands — click a band to select it (Ctrl/Cmd+click for several), drag its handles to adjust, click ✕ to delete spurious ones, mark the ladder lane or click "No ladder".
- For normalization: switch to the Housekeeping membrane (toggle above) and Analyze it too.
- Assign band roles / read ÷HK ratios.
- Optional: calibrate kDa from the ladder.
🧬
Drop Western Blot image here
or click to browse
Inspector
Step 1 of 4
Load a Western Blot image to begin.
AI summary
conf · -
Analysis results will appear here after processing.
LADDER
Raw int.
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Bg-subtracted
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÷GAPDH
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vs DMSO
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Saturated band
Guided: Click a band overlay on the blot to see its details. Ctrl/Cmd+click to select several.
0 bands selected
Calibration
R²: -
Rungs paired: -
Select a ladder lane and assign kDa values.
Lanes
0
Guided: Ladder lane is auto-detected. Click "Set as ladder" to change.
Fold-change vs DMSO
Statistics
Valid bands
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Anomalies
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Expert Details
Pipeline latency:
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Segmentation:
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Quantification:
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Scene type:
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Aspis Helper
blot-aware
AI